Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities

Research conducted to meet the needs of standard compounds in practical and research activities. The use improves quality a study. Piperine is main secondary metabolite compound alkaloid group contained Black Pepper Piper nigrum L. isolation method used reflux recrystallization methods. process absorbing piperine with diethyl ether solvent will help break down cell walls absorb optimally. Recrystallization was repeated 7-8 times until crystals were pale yellow. purity test TLC ratio n-hexane chloroform 6:4 an Rf value 0.04 cm. GCMS confirmation 85% purity, UV-Visible spectroscopic maximum wavelength 343 absorbance 0.865 FTIR confirmed wave number 928 cm-1 (CO), 996 ((CH bending trans –CH=CH-), 1028 cm-1(=COC sym. st.), 1250 (=COC asym. 1440 (methylenedioxy CH2bending), 1581 (arom. C=C 1631 (-CO-N-). Based on results, isolated from group. can be as preparations for testing chemistry lab work natural ingredients.